The study by Vantaux and colleagues evaluated a broad spectrum of mosquito fitness traits that may be impacted by human infections with Plasmodium falciparum and host choice in subsequent blood meals. Through extensive experimental challenges using four different blood sources in sequential feedings, the authors found that Plasmodium-infected mosquitoes had lower feeding rates compared to uninfected mosquitoes, an effect observed for all four blood types in the first feeding after Plasmodium exposure. Infection status did not affect any other trait, which were largely influenced by the host species of the blood source. Additionally, the blood source did not affect Plasmodium development within the mosquito. These results provided essential parameters to model vectorial capacity in multi-host environments.

The introduction is comprehensive and provides a clear rational behind the study. The manuscript is fairly well-written. However, bellow I have addressed some parts that could be further clarified. The laboratory methods used to evaluate the fitness traits are reliable, and the statistical analyses performed are robust .

The authors used field isolates of P. falciparum and lab populations of An. coluzzii that were frequently replenished with wild-caught F1 mosquitoes, making the experimental set-up similar, but not identical to "natural" conditions. This is a major contribution of this study, but it would be important that the authors provide evidence that the replenishment of lab colonies with wild mosquitoes if sufficient to remove inbreeding effects that may affect mosquito-Plasmodium interactions. I am saying this because a substantial portion of the first paragraph of the 'Discussion' is dedicated to comparisons between natural and non-natural experiments. Although this was addressed in the "study limitations" section, the authors could tone down the assumption that they used a natural system.

It would be important to include the effect of blood meal size on the models evaluating mosquito survival, fecundity, development time and F1 wing length since the amount of blood ingested may play a role in these traits.

It is unclear why the authors kept mosquitoes in cages for trials 3-5. Was it for convenience since the traits measured in these trials did not require close observation of individual mosquitoes? In any case, this could be clarified early in the 'Methods' section.

It's very interesting that feeding rate was the only trait affected by Plasmodium infection. However, does the experimental set-up (artificial feeding) allow authors to extrapolate this effect to real-world scenarios? I wonder if it would be better to use feeding-rate data from studies using whole-body to address feeding success. Even studies using immobilized hosts may provide more realistic information compared to the present study that used parafilm membrane for feeding. Using these parameters for the modeling is probably fine, though, since the cross-comparisons among blood types provide the relative impact of each host species.

The authors explain only in the 'Discussion' why they did not sugar-fed mosquitoes in their experiments. It may be worth explaining this in the 'Methods' as well. In the 'Discussion', it would be interesting to see some explanation about how sugar-feeding may alleviate potential fitness costs in infected mosquitoes. Has this been demonstrated before? Ferguson et al 2003 (https://doi.org/10.1111/j.0014-3820.2003.tb01521.x) provide evidence that sugar restriction reduces survival in infected mosquitoes.

L314 - Maybe the authors could clarify which prevalence is represented in the pie charts in Fig. 1, whether it’s the oocyst or sporozoite rates.

L503-504 - What did authors mean by "that by not separating exposed-infected and exposed-uninfected females we were not able to measure them". Mosquito groups exposed to either infected or uninfected blood meals were mixed in the same cup/cage? Please clarify this in the 'Methods' and/or in the 'Discussion'.

The article entitled "Multiple hosts, multiple impacts: the role of vertebrate host diversity in shaping mosquito life history and pathogen transmission" provides valuable information on the effect of blood-meals on the physiology, reproduction, survival and epidemiology of Anopheles coluzzi, particularly in the gonotrophic cycle. The authors acknowledge the limitations of a laboratory study, but provide bases for studies in natural environments. Therefore, I recommend being accepted for publication in this journal.

It is a complete and interesting article, I only have one suggestion:

Figure 3 and Figure 6.- The colors cause me confusion, I recommend that in graphs B and D use gray scale or include the four solid or degraded colors in exposed and unexposed.

Figure 2.- It provides relevant information, but it is small. I recommend separating or enlarging, to facilitate reading.